nancy strain  (ATCC)

Journal: Scientific Reports

Article Title: Impact of NSAIDs and endurance exercise on myocardial fibrosis and arrhythmogenesis in murine coxsackieviral myocarditis

doi: 10.1038/s41598-025-13437-x

Figure Lengend Snippet: Myocardial inflammation at sacrifice. ( A ) Representative morphological images of the inflammatory lesions in the heart during the acute (D7) and chronic (D41-45) stages of coxsackievirus B3-induced myocarditis. Hematoxylin and eosin staining. Scale bar = 100 μm. ( B ) Semiquantitative scoring of myocardial inflammation. Monte Carlo chi-square testing indicated no significant differences between groups ( P = .567). ( C ) Quantitative assessment of the area of inflammation in the myocardium. Kruskal-Wallis H testing revealed no significant differences between groups in the percentage of inflammation area ( P = .493). ( D ) Cardiac CVB viral load at sacrifice was measured by dPCR. Kruskal Wallis H testing indicated no statistically significant differences between groups ( P = .860). For reference, median viral load values from a subset of animals sacrificed 7 days post-inoculation are also included ( P = .494). ( E–G ) Cardiac gene expression at sacrifice was assessed by qPCR. Messenger RNA levels are expressed as fold change relative to the CVB-SED group and normalized to GAPDH and CDK1NB. For TNFα, a two-way ANOVA with Tukey’s post-hoc test was performed, while statistical differences for IL-1β and IL-6 were evaluated using Kruskal-Wallis H testing followed by Dunn’s multiple comparisons. Data are presented as ( B ) frequency histograms or as ( C – G ) boxplots with interquartile ranges. Group sizes for panels ( B – G ) CVB-SED: n = 12–17, CVB-EEX: n = 10–16, CVB-NSAID-SED: n = 11–17, CVB-NSAID-EEX: n = 9–14.

Article Snippet: The human coxsackievirus B3 strain Nancy was obtained from the American Tissue Culture Collection (France), cultured, and quantified using a plaque assay.

Techniques: Staining, Gene Expression

Journal: Scientific Reports

Article Title: Impact of NSAIDs and endurance exercise on myocardial fibrosis and arrhythmogenesis in murine coxsackieviral myocarditis

doi: 10.1038/s41598-025-13437-x

Figure Lengend Snippet: Myocardial fibrosis at sacrifice. ( A ) Representative morphological images of fibrotic lesions and scars in the heart during the chronic stage of coxsackievirus B3-induced myocarditis. Picrosirius red staining. Scale bar = 100 μm. ( B ) Semiquantitative scoring of myocardial fibrosis and scarring. Monte Carlo chi-square testing indicated no statistically significant differences in the extent of perivascular fibrosis ( P = .273), interstitial fibrosis ( P = .677), or myocardial scarring ( P = .534) among the groups. ( C ) The myocardial scar counts and ( D ) collagen surface area, assessed by picrosirius red staining, were comparable between groups ( P = .473 and P = .350, respectively). ( E – G ) Cardiac gene expression at sacrifice was assessed by qPCR. Messenger RNA levels are expressed as fold change relative to the CVB-SED group and normalized to GAPDH and CDK1NB. For genes CTGF and COL1A1, a two-way ANOVA with Tukey’s post-hoc test was performed, while statistical differences for COL3A1 were evaluated using Kruskal-Wallis H testing followed by Dunn’s multiple comparisons. Data are presented as ( B ) frequency histograms or as ( C – G ) boxplots with interquartile ranges. Group sizes for panels ( B – G ) CVB-SED: n = 12–17, CVB-EEX: n = 16, CVB-NSAID-SED: n = 17, CVB-NSAID-EEX: n = 14.

Article Snippet: The human coxsackievirus B3 strain Nancy was obtained from the American Tissue Culture Collection (France), cultured, and quantified using a plaque assay.

Techniques: Staining, Gene Expression

Journal: Journal of Medicinal Chemistry

Article Title: Design of a Fluorescence Polarization Probe for Enterovirus 2C Proteins

doi: 10.1021/acs.jmedchem.5c01219

Figure Lengend Snippet: Genome structure of enterovirus, structure of 2C, and reported 2C inhibitors. (A) The EV genome contains ORF encoding structural proteins VP1, VP2, VP3, and VP4 and nonstructural proteins 2A, 2B, and 2C and 3A, 3B, 3C, and 3D. (B) Alignment of the crystal structure of CVB3 2C in complex with SFX (PDB ID 6S3A , colored in gray) and EV-A71 2C in complex with ATPγS (PDB ID 5GRB , colored in wheat). The C-terminal part of 2C is highlighted in orange, the zinc finger domain is highlighted in green, ATPγS is shown in blue sticks, and SFX is shown in yellow sticks. (C) Chemical structures of reported enterovirus 2C inhibitors.

Article Snippet: The enterovirus strains used in this study were obtained from commercial sources: EV-D68 US/MO/14-18947 (ATCC, NR-49129), EV-A71 Tainan/4643/1998 (BEI Resources, NR-471), and CVB3 strain Nancy (ATCC, VR-30).

Techniques:

Journal: Journal of Medicinal Chemistry

Article Title: Design of a Fluorescence Polarization Probe for Enterovirus 2C Proteins

doi: 10.1021/acs.jmedchem.5c01219

Figure Lengend Snippet: Design of the 2C FP probe. (A) FP assay principle. (B) SAR study and design of the 2C FP probe Jun14157 . (C) Superimposed structures of EV-D68 2C (homology model) with CVB3 2C in complex with SFX (PDB ID 6T3W ). EV-D68 2C is colored in green, CVB3 2C is colored in magenta, and the cavity of the allosteric pocket is colored in cyan. SFX is shown as wheat sticks. (D) The superposition of docking poses of Jun571 , Jun1377 , and Jun14157 occurred at the allosteric site of EV-D68 2C. 2C is colored in split pea, Jun571 is shown as gray sticks, Jun1377 is shown as marine sticks, and Jun14157 is shown as pink sticks.

Article Snippet: The enterovirus strains used in this study were obtained from commercial sources: EV-D68 US/MO/14-18947 (ATCC, NR-49129), EV-A71 Tainan/4643/1998 (BEI Resources, NR-471), and CVB3 strain Nancy (ATCC, VR-30).

Techniques: FP Assay

Journal: Journal of Medicinal Chemistry

Article Title: Design of a Fluorescence Polarization Probe for Enterovirus 2C Proteins

doi: 10.1021/acs.jmedchem.5c01219

Figure Lengend Snippet: Optimization of the FP assay using the Jun14157 probe. (A) Binding curves of the Jun14157 probe at 20, 50, and 100 nM with increasing concentrations of EV-D68 2C after 30 min of incubation. K d values represent means ± standard deviation (SD) from triplicate experiments. (B) Effect of dimethyl sulfoxide (DMSO) concentration on the mP values of bound and free probes. The assay was performed in triplicate. (C) Binding curves of 50 nM Jun14157 with increasing concentrations of WT and EV-D68 2C mutants D183V, D323G, and D183V/D323G. K d values are the mean ± SD from duplicate experiments. (D–F) FP titration curves of Jun14157 with EV-D68 2C-WT (D), EV-A71 2C (E), and CVB3 2C (F) with different incubation times. K d values are presented as the mean ± SD from triplicate experiments. Each data point represents the mean ± SD.

Article Snippet: The enterovirus strains used in this study were obtained from commercial sources: EV-D68 US/MO/14-18947 (ATCC, NR-49129), EV-A71 Tainan/4643/1998 (BEI Resources, NR-471), and CVB3 strain Nancy (ATCC, VR-30).

Techniques: FP Assay, Binding Assay, Incubation, Standard Deviation, Concentration Assay, Titration